Thème :

Surveillance épidémiologique et prévention des maladies infectieuse

Type de présentation :

Présentation Orale

Titre abstract :

Prevalence of antibodies specific to HIV envelope gp41 derived membrane proximal external region (MPER) in HIV-1 discordant couples of the Ngaoundere Regional Hospital Cameroon

Auteurs :

Sonia Nina MENGUE NDJANA^1*, Alfred APEH NGOH¹, TAKOUA Doline¹, George OKECHUKWU CHUKWUMA¹, Suzanne hippolite MAGAGOUM¹, Lissom LISSOM¹, Ghislain Donald NJAMBE PRISO¹, Dieudonné NDJONKA¹, Godwin NCHINDA¹, Lovelyne NGU ¹

 

 

Institutions:

¹Université de Ngaoundéré, Cameroun

Corresponding authors :

sonimani1505@yahoo.fr

Référence :

CaHReF 2018, Yaoundé Congres hall, 08 – 11 January 2019 , OSEP032

Abstract :

Background: The MPER is a desirable vaccine immunogen because as conserved region of the HIV envelope it is not only targeted by several broadly neutralizing antibodies but also necessary for viral infectivity. Thus MPER specific antibodies in plasma from highly exposed uninfected people could predict their role in preventing seronegative partners

Objectif: Contribute to the development of an HIV-1 vaccine

Methodolody: Briefly 4ml of blood in EDTA tubes was obtained from consented confirmed HIV-1 discordant couples visiting the Ngaoundere regional hospital HIV management center under ethical approved protocol number 2018/03/991/CE/CNERSH/SP. Plasma was harvested subsequently transported frozen (-20C) to the CIRCB laboratory of Vaccinology and Biobanking in Yaoundé. After performing HIV confirmatory test using the Genius biorad system we next tested in an ELISA assay for the presence of MPER specific antibodies in participants plasma. Here a recombinant QβMPER phage displaying several molecules of MPER upon its surface was used as antigen for coating the ELISA plates

Results: Our study showed a significantly higher prevalence of MPER-specific IgM antibodies in seronegative partners (70%) compared to theirfor HIV positive partners (30%). Similarly for MPER-specific IgG antibodies, seronegative partners had a prevalence of 52%, while their seropositive partners had a prevalence of 48%. With respect to MPER-specific IgA antibodies seronegative partners showed a significantly higher prevalence (85%) compared to 15% mfor the HIV positive partners

Conclusion/Recommandation: Thus seronegative partners of HIV discordant couples showed significantly higher prevalence of MPER specific antibodies than their positive conterpart. This probably suggest such antibodies might protect them from acquiring HIV

Key Words: HIV-1, Neutralizing Antibodies, MPER, Discordant pairs