In vitro and in vivo antisalmonellal potential of leaf extracts of Tristemma mauritianum and his effects on haematological parameters in wistar rats

CaHReF 2016, Yaoundé Conges hall, 23 – 26 August 2016 , PP20

Typhoid fever and others salmonellosis remain a marked problem of public health in the world. The most affected continents are Africa and Asia. Since the causative agents rapidly become resistant to common antibiotics, 
Objective: This work aimed to evaluate the in vitro and in vivo the potential of extracts and fractions of Tristemma mauritianum against bacteria of Salmonella. 

The methanol and its fractions as well as the water extracts of Tristemma mauritianum were tested for in vitro antisalmonellal activity using broth microdilution technique. The methanol extract (most active crude extract) was tested in vivo against salmonella-induced thyphoid in Rats. 

Among crude extracts, the methanol extract showed the best in vitro activity with MICs varying from 96-192 µg/ml. While the ethyl acetate fraction presented the best inhibitory activity with MICs ranging from 48-192 µg/ml. Nevertheless, this activity was less than that exerted by the reference drugs (MICs = 0.375-0.1875 µg/ml). In vivo, the activity of the methanol extract was comparable to the one of oxytetracycline (the reference drugs) since both test substances were able to heal infected rats after six days of treatment with 20 mg/kg bw. 
Conclusion: The results of this work provide the baseline information for the possible used of the Tristemma mauritianum to treat typhoid fever and others salmonellosis.

David Ngoudjou Tsafac; Donatien Gatsing ; Guy Sedar Singor Njateng