Steve EKOM ENDEGUELE1
1Université of Dschang, Cameroon.
CaHReF 2018, Yaoundé Congres hall, 08 – 11 January 2019 , OAU013
Background: Bacterial infection remaina major threat to humain health worldwide. Wound infections are among the major and widely occuring pathology. Staphylococcus aureus is the most common cause of wound infection. This bacterial bas been reported to have develop resistance toward antibiotic. P. guajava bas been reported to have antibacterial activity.
Objectif: to investigate the antibacterial activity and healing efficacy of Psidium guajava leaf on an excision-wound infected with Staphylococcus aureus using a rat model.
Methodology: The antibacterial activities of the methanol leaf extract alone and combinations of the plant extract with amoxicillin as well as the effect of osmotic stress were determined by using broth microdilution method. The therapeutic effect of the methanol extract was evaluated on an excision-wound infected with Staphylococcus aureus
Results: The plant extract displayed antibacterial activity (MIC = 256 - 1024 Î¼g/ml) that varied according to the tested bacterial species. Synergistic effect between amoxicillin and P. guajava extract was observed. The antibacterial activity of the plant extract and chloramphenicol increased under osmotic stress condition whereas that of amoxicillin decreased under this condition. P. guajava extract and Baneocin ointments gave the shortest epithelization times and highest wound contraction rates as well as the greatest weights and total protein contents of granulation tissues as compared to the negative control. The P. guajava methanol extract ointment is non-irritating to the skin and slightly
Conclusion/Recommandation: The results of the present study demonstrate the wound healing and antibacterial properties of P. guajava and confirm its traditional use in the treatment of wounds and infectious diseases.
Key Words: Psidium guajava, wound healing, antibacterial, toxicity.