Marie Nicole NGOUFACK1*, Patrice DJATAOU1, Céline NGUEFEU NKENFOU1.
1University of Yaounde I, Cameroon
CaHReF 2018, Yaoundé Congres hall, 08 – 11 January 2019 , OAEI009
Background: Chemokines and their receptors have been implicated as pivotal players in many pathological and physiological conditions including their role as coreceptors in the transmission of HIV.
Objectif: To determine the frequency of CCR5-delta 32, CCR2-64I and SDF1- 3'A genes polymorphism and to look at their association with HIV infection.
Methodology: This was an epidemical analytic case-control study carried out in the Regional Hospital of Ngaoundere. A total of 217 participants were recruited. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to analyze genotypes CCR5-delta 32, CCR2-64I and SDF1- 3'A of these participants. Data obtained were analyzed using Hardy-Weinberg equilibrium.
Results: Among 217 participants enrolled, 65 (29.95%) were HIV positive and 152 (70.05%) were HIV negative. The allelic frequencies obtained were 0.0%, 17.75% and 43.78% for CCR5-delta 32, CCR2-64I and SDF1- 3'A genes respectively. The allelic frequency of CCR2-64I in the negative participants were significantly higher than in HIV positives participants (P=0.0049) but was not the case of SDF1-3'A gene (P=0.19)
Conclusion/Recommandation: The polymorphism of CCR2-64I may contribute to protecting patients against HIV infection. Chemokines receptors genotyping should be useful routinely in predicting the outcome of hiv-1 transmission upon exposure. Preventing HIV transmission should be reinforced in persons with mutated CCR2 gene.
Key Words: HIV, Frequency, AIDS related genes, Adamawa-Cameroon